ABSTRACT
Objective To investigate the expression and clinical significance of survivin and PTEN in bladder transitional cell carcinoma(TCC).Methods The expression of survivin and PTEN was studied by S-P immunohistochemisty in 10 cases of normal urinary bladder tissues and 62 cases of the bladder TCC. Results All the normal urinary bladder tissues showed positive staining pattern of PTEN protein, negative staining pattern of survivin protein, positive rate of PTEN in 62 cases of the bladder TCC is 46.8%,the difference of positive rate between grade Ⅰ and grade Ⅲ was significant(P＜0.05),the difference of positive rate between Tis～T_1 and T_2～T_4 showed a trend of decreasing, but there was no statistically significant difference(P＞0.05);positive rate of survivin in 62 cases of the bladder TCC is 56.5%,the difference of positive rate between grade Ⅰ and grade Ⅲ was significant(P＜0.05),the difference of positive rate between Tis～T_1 and T_2～T_4 was significant too(P＜0.05).The expression of PTEN protein and survivin protein was reversely correlated(P＜0.05).Conclusion The abnormal expression of PTEN protein and survivin protein play an important role in the occurrence and progress of the bladder TCC.It can be regarded as a useful diagnostic marker in the bladder TCC.
ABSTRACT
Objective To establish an animal model to investigate the effects of prolonged vaginal distension on the leak-point pressure(LPP) and urethral anatomy.Methods Eighteen virgin female rats were randomly divided into control group(6 rats) and experimental group(12 rats).In the experimental group,the rats were underwent vaginal distension for 4 h. The rats in the control group were not received vaginal distension.The greatest bladder volume and LPP were measured,and sneeze test was performed for all rats in two groups 4 weeks after distension.All the rats were killed after the test and the bladder and urethra were harvested for histological study.Results The greatest bladder volume and LPP were significantly decreased than those of control group.Stress urinary incontinence(SUI) was occurred in 45.5% rats of the experiment group,but none in control group.Histological studies showed that the urethral striated and smooth muscle became thin,but the collagenic tissue was increased.There was no change in control group.Conclusion Prolonged vaginal distension can cause SUI and it is a desirable animal model for studying the mechanism and treatment of SUI.